CNN3 Polyklonaler Antikörper

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• KD/KO Validated

CNN3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11509-1-AP

Synonyme

CNN 3, Calponin-3, Calponin3, Calponin 3

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11509-1-AP

  WB result of CNN3 antibody (11509-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN3 transfected HEK-293 cells.

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  WB analysis using 11509-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 cells using 11509-1-AP

  HAP1 (WT and CNN3 KO) lysates prepared with RIPA buffer, 20 μg of protein loaded. 11509-1-AP incubated at 1:100 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of human kidney using 11509-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11509-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of U-87MG cells using 11509-1-AP

  U-87MG lysates prepared and IP of APP performed using 2.0 μg of 11509-1-AP coupled to protein A- Dynabeads beads. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitated; HC = antibody heavy chain. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of HEK-293 using 11509-1-AP

  IP result of anti-CNN3 (IP:11509-1-AP, 4ug; Detection:11509-1-AP 1:15000) with HEK-293 cells lysate 960 ug.

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  IHC staining of human prostate cancer using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hysteromyoma using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hysteromyoma using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11509-1-AP

  Immunofluorescent analysis of Hela cells, using CNN3 antibody 11509-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Nierengewebe, humanes Skelettmuskelgewebe, NIH/3T3-Zellen
• Erfolgreiche IP: HEK-293-Zellen, U-87MG cells
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Hautgewebe, humanes Magenkrebsgewebe, humanes Uterusmyomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11509-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CNN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNN3 fusion protein Ag2068
• Vollständiger Name: calponin 3, acidic
• Berechnetes Molekulargewicht: 329 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC025372
• Gene symbol: CNN3
• Gene ID (NCBI): 1266
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Cell Rep; Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.; Authors - Taha A Jan
human	WB	J Proteome Res; Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma; Authors - Pei-Shan Wu
mouse	IHC	Mol Cell Biochem; STAT3 mediates ECM stiffness-dependent progression in ovarian cancer; Authors - Sun Chenchen
human	WB	Gastroenterol Res Pract; PMID: 30911294; Authors - Keun-Seok Hong ; KD Validated