Calponin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Calponin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

45

Rezensionen (2)

Kat-Nr. : 13938-1-AP

Synonyme

CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin

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Galerie der Validierungsdaten

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  WB analysis using 13938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13938-1-AP (Calponin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13938-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13938-1-AP (CNN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13938-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13938-1-AP (CNN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13938-1-AP

  IP result of anti-Calponin (IP:13938-1-AP, 4ug; Detection:13938-1-AP 1:1000) with mouse lung tissue lysate 2800ug.

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  IHC staining of human heart using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human appendicitis using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 13938-1-AP (Calponin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13938-1-AP (CNN1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13938-1-AP (CNN1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human hysteromyoma using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 13938-1-AP (CNN1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hysteromyoma using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 13938-1-AP (CNN1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human bladder using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hysteromyoma using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hysteromyoma using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13938-1-AP (CNN1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 13938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13938-1-AP (CNN1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse heart using 13938-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 13938-1-AP (Calponin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C2C12 using 13938-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (13938-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C2C12 using 13938-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (13938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of C2C12 using 13938-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Calponin (13938-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, Mausherzgewebe, Mauslungengewebe, Maus-Uterusgewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe, humanes Blasengewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Uterusmyomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen
• Erfolgreiche Detektion in FC (Intra): C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 31 publications below
• IHC: See 4 publications below
• IF: See 13 publications below

Produktinformation

13938-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Calponin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calponin fusion protein Ag4989
• Vollständiger Name: calponin 1, basic, smooth muscle
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC022015
• Gene symbol: Calponin
• Gene ID (NCBI): 1264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.; Authors - Xue Gong
rat	IF	Acta Biomater; Hybrid electrospun rapamycin-loaded small-diameter decellularized vascular grafts effectively inhibit intimal hyperplasia.; Authors - Yang Yang
mouse	WB	Theranostics; Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.; Authors - Gao-Shan Yang
rat	WB	Phytomedicine; Betulinaldehyde inhibits vascular remodeling by regulating the microenvironment through the PLCγ1/Ca2+/MMP9 pathway; Authors - Yangxia Fu
mouse	WB	Arterioscler Thromb Vasc Biol; CRISPR-Cas9 genome editing of a single regulatory element nearly abolishes target gene expression in mice--brief report.; Authors - Yu Han
human	WB	Genomics; Identification of transfer RNA-derived fragments and their potential roles in aortic dissection.; Authors - Xiuxiu Fu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yajing (Verified Customer) (11-12-2019)

The antibody works well. Can be reused when stored at 4 degrees.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HPASMC

Calponin Antibody Western Blot, validation (1:1000 dilution) in HPASMC (Cat no:13938-1-AP)

FH

Ying (Verified Customer) (08-19-2019)

very good antibody

• Applications: Western Blot,
• Cell Tissue Type: mouse primary cardiomyocytes

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C