CNKSR1 Polyklonaler Antikörper

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• KD/KO Validated

CNKSR1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10885-1-AP

Synonyme

CNK, CNK homolog protein 1, CNK1, CNKSR1, Connector enhancer of KSR 1, Connector enhancer of KSR like, hCNK1, KSR

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Galerie der Validierungsdaten

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  WB analysis of HT-29 using 10885-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 10885-1-AP (CNKSR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10885-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10885-1-AP (CNKSR1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HT-29 using 10885-1-AP

  IP result of anti-CNKSR1 (IP:10885-1-AP, 4ug; Detection:10885-1-AP 1:1000) with HT-29 cells lysate 4000 ug.

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  IHC staining of human pancreas cancer using 10885-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10885-1-AP (CNKSR1 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10885-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10885-1-AP (CNKSR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, humanes Hirngewebe
• Erfolgreiche IP: HT-29-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Produktinformation

10885-1-AP bindet in WB, IP, IHC, ELISA CNKSR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNKSR1 fusion protein Ag1333
• Vollständiger Name: connector enhancer of kinase suppressor of Ras 1
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC011604
• Gene symbol: CNKSR1
• Gene ID (NCBI): 10256
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CNKSR1 is a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. CNKSR1 is required in multiple receptor tyrosine kinase pathways where it appears to be a tyrosine phosphorylation targe.

Publikationen

Species	Application	Title
human	WB,IHC	Life Sci Alliance; CNKSR1 serves as a scaffold to activate an EGFR phosphatase via exclusive interaction with RhoB-GTP.; Authors - Kanako Nishiyama ; KD Validated
human	IHC	Cancer Rep (Hoboken); Identification of hub genes in bladder cancer based on weighted gene co-expression network analysis from TCGA database.; Authors - Lei Wang
human	IHC	Heliyon; Identification of CNKSR1 as a biomarker for "cold" tumor microenvironment in lung adenocarcinoma: An integrative analysis based on a novel workflow; Authors - Qidong Cai