UMP/CMP kinase Polyklonaler Antikörper

UMP/CMP kinase Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11360-1-AP

Synonyme

CMPK1, Cytidylate kinase, Cytidine monophosphate kinase, CMPK, CMK

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Galerie der Validierungsdaten

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  WB analysis of mouse pancreas using 11360-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11360-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11360-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 11360-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11360-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, humanes Pankreasgewebe, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11360-1-AP bindet in WB, IHC, IF/ICC, ELISA UMP/CMP kinase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UMP/CMP kinase fusion protein Ag1920
• Vollständiger Name: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Berechnetes Molekulargewicht: 228 aa, 26 kDa
• Beobachtetes Molekulargewicht: 22-26 kDa
• GenBank-Zugangsnummer: BC014961
• Gene symbol: CMPK1
• Gene ID (NCBI): 51727
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.

Publikationen

Species	Application	Title
		J Proteomics; Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry.; Authors - Víctor Llombart