CLSTN3 Polyklonaler Antikörper

CLSTN3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IP, CoIP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13302-1-AP

Synonyme

calsyntenin 3, Alc-beta, alcbeta, Alcadein-beta, Alcadein beta

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Galerie der Validierungsdaten

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  WB analysis using 13302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13302-1-AP (CLSTN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 13302-1-AP

  pig brain tissue were subjected to SDS PAGE followed by western blot with 13302-1-AP (CLSTN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13302-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13302-1-AP (CLSTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13302-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13302-1-AP (CLSTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Hausschwein-Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13302-1-AP bindet in WB, IHC, IP, CoIP, ELISA, IF CLSTN3 und zeigt Reaktivität mit Hausschwein, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLSTN3 fusion protein Ag4107
• Vollständiger Name: calsyntenin 3
• Berechnetes Molekulargewicht: 968 aa, 107 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa
• GenBank-Zugangsnummer: BC039075
• Gene symbol: CLSTN3
• Gene ID (NCBI): 9746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calsyntenins, also called alcadeins, are cadherin superfamily proteins first identified as synaptic proteins (PMID: 11161476; 12498782). Calsyntenins are type I transmembrane proteins with extracellular domains containing two cadherin repeats and an LNS (laminin, neurexin, sex hormone-binding globulin) domain (PMID: 24613359). Calsyntenin-3 (CLSTN3, also known as alcadein-beta) is a synapse-organizing protein that promotes the development of synapses. It is a postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation (PMID: 25352602; 24613359).

Publikationen

Species	Application	Title
mouse	WB,IP	Nature; CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization; Authors - Kevin Qian
human,mouse	WB,IF	Mol Metab; CLSTN3 gene variant associates with obesity risk and contributes to dysfunction in white adipose tissue.; Authors - Ningning Bai
human	WB,CoIP	Int J Biol Macromol; Calsyntenin-3 interacts with the sodium-dependent vitamin C transporter-2 to regulate vitamin C uptake; Authors - Veedamali S. Subramanian
mouse	WB	Neuroscience; CircAFF2 promotes neuronal cell injury in intracerebral hemorrhage by regulating the miR-488/calsyntenin-3 axis; Authors - Juxing Qi ; KD Validated
mouse	WB	Int J Mol Sci; Transcriptome Profile in the Mouse Brain of Hepatic Encephalopathy and Alzheimer’s Disease; Authors - Young-Kook Kim