CLIC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CLIC1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 14545-1-AP

Synonyme

Chloride channel ABP, CLIC1, G6, hRNCC, NCC27

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 14545-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14545-1-AP (CLIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14545-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14545-1-AP (CLIC1 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14545-1-AP

  IP Result of anti-CLIC1 (IP:14545-1-AP, 4ug; Detection:14545-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IHC staining of human small intestine using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14545-1-AP

  Immunofluorescent analysis of HepG2 cells, using CLIC1 antibody 14545-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 14545-1-AP

  Immunofluorescent analysis of Hela cells, using CLIC1 antibody 14545-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Kolongewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

14545-1-AP bindet in WB, IP, IHC, IF, ELISA CLIC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLIC1 fusion protein Ag6049
• Vollständiger Name: chloride intracellular channel 1
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27-32 kDa
• GenBank-Zugangsnummer: BC064527
• Gene symbol: CLIC1
• Gene ID (NCBI): 1192
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	IF	Oxid Med Cell Longev; Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.; Authors - Songhan Li
human	WB	Transl Oncol; Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling; Authors - Huiwu Geng ; KD Validated
		Cell Biochem Biophys; CLIC1 Inhibition Protects Against Cellular Senescence and Endothelial Dysfunction Via the Nrf2/HO-1 Pathway.; Authors - Dezhao Lu
human	IHC	Anticancer Res; Chloride Intracellular Channel 1 Expression Is Associated With Poor Prognosis of Lung Adenocarcinoma.; Authors - Yuichiro Yasuda
mouse	WB,IHC	Technol Cancer Res Treat; CLIC1 Drives Angiogenesis in Hepatocellular Carcinoma by Modulating VEGFA.; Authors - Xuyong Wei ; KO Validated
human	WB,IHC	Breast Cancer (Dove Med Press); Chloride Intracellular Channel 1 is a Potential Biomarker for Breast Cancer; Authors - Jinwen Xia

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