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Claudin 9-specific Polyklonaler Antikörper

Claudin 9-specific Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16196-1-AP

Synonyme

claudin 9, CLDN9

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of HeLa cells using Claudin 9-specific Polyclonal antibody (16196-1-AP)

    WB analysis of HeLa using 16196-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16196-1-AP (Claudin 9-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Claudin 9-specific Polyclonal antibody (16196-1-AP)

    IP experiment of HeLa using 16196-1-AP

    IP Result of anti-Claudin 9-specific (IP:16196-1-AP, 4ug; Detection:16196-1-AP 1:300) with HeLa cells lysate 880ug.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen
Erfolgreiche IPHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 2 publications below
IHCSee 1 publications below

Produktinformation

16196-1-AP bindet in WB, IP, IHC,ELISA Claudin 9-specific und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name claudin 9
Berechnetes Molekulargewicht 23 kDa
Beobachtetes Molekulargewicht 25-29 kDa
GenBank-ZugangsnummerNM_020982
Gene symbol CLDN9
Gene ID (NCBI) 9080
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 9.

Protokolle

Produktspezifische Protokolle
WB protocol for Claudin 9-specific antibody 16196-1-APProtokoll herunterladen
IP protocol for Claudin 9-specific antibody 16196-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratWB, IHC

Histochem Cell Biol

Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.

Authors - Kratzer Ingrid I
View Article
humanWB

Front Genet

Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma.

Authors - Zezhong Mou
View Article