CKMT2 Polyklonaler Antikörper

CKMT2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 13207-1-AP

Synonyme

CKMiMi, CKMT2, Mib CK, S MtCK, SMTCK

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Galerie der Validierungsdaten

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  WB analysis using 13207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13207-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 13207-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

13207-1-AP bindet in WB, IHC, ELISA CKMT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CKMT2 fusion protein Ag3971
• Vollständiger Name: creatine kinase, mitochondrial 2 (sarcomeric)
• Berechnetes Molekulargewicht: 419 aa, 48 kDa
• Beobachtetes Molekulargewicht: 41-48 kDa
• GenBank-Zugangsnummer: BC029140
• Gene symbol: CKMT2
• Gene ID (NCBI): 1160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity.; Authors - Suzhen Chen
chicken	WB	Mol Cell Proteomics; Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens; Authors - Yunlei Li
mouse	WB,IHC	Metabolism; Sustained mitochondrial biogenesis is essential to maintain caloric restriction-induced beige adipocytes.; Authors - Raja Gopal Reddy Mooli
human	WB	Front Cell Dev Biol; Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma; Authors - Wenshuo Zhang
chicken	WB	Mol Cell Proteomics; Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens.; Authors - Yunlei Li
mouse	WB	J Agric Food Chem; Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.; Authors - Sho Nishikawa