CKM-Specific Polyklonaler Antikörper

CKM-Specific Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18712-1-AP

Synonyme

CKMM, Creatine Kinase MM, Creatine Kinase M-Specific, Creatine kinase M chain, Creatine kinase M type, creatine kinase, muscle, M CK

---

Galerie der Validierungsdaten

• 

  WB analysis using 18712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18712-1-AP (CKM-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 18712-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 18712-1-AP (CKM-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18712-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18712-1-AP (CKM-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18712-1-AP (CKM-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18712-1-AP (CKM-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart using 18712-1-AP (CKM-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat heart using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart using 18712-1-AP (CKM-Specific antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Dünndarmgewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

18712-1-AP bindet in WB, IHC, ELISA CKM-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Bakterium
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: creatine kinase, muscle
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC007462
• Gene symbol: CKM
• Gene ID (NCBI): 1158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42 kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185). This antibody is specific to CKM.

Publikationen

Species	Application	Title
	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
mouse	WB	Cell Prolif; KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.; Authors - Jing Hao
mouse	WB	Toxicology; Pb inhibited C2C12 myoblast differentiation by regulating HDAC2; Authors - Xiaozhen Gu
Bacterial	WB	Mol Pharmacol; Naturally Occurring Mutations to Muscle-Type Creatine Kinase Impact Its Canonical and Pharmacological Activities in a Substrate-Dependent Manner In Vitro.; Authors - Eric P Mosher
mouse	WB	BMC Musculoskelet Disord; Ang-(1-7) protects skeletal muscle function in aged mice.; Authors - Ying Li