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CIN85 Polyklonaler Antikörper

CIN85 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12132-1-AP

Synonyme

CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBRaji-Zellen, fetales humanes Hirngewebe, Jurkat-Zellen, NIH/3T3-Zellen, Ramos-Zellen, THP-1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12132-1-AP bindet in WB, IF, IHC, ELISA CIN85 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CIN85 fusion protein Ag2777
Vollständiger Name SH3-domain kinase binding protein 1
Berechnetes Molekulargewicht 665 aa, 73 kDa
Beobachtetes Molekulargewicht 85 kDa
GenBank-ZugangsnummerBC015806
Gene symbol SH3KBP1/CIN85
Gene ID (NCBI) 30011
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

Protokolle

Produktspezifische Protokolle
WB protocol for CIN85 antibody 12132-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB, IF

J Cell Biol

CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface.

Authors - Ihor Yakymovych
humanWB

Biol Cell

ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking.

Authors - Yoon Hye-Young HY
  • KD Validated
humanIHC

Arch Gynecol Obstet

Significance of PTPRZ1 and CIN85 expression in cervical carcinoma.

Authors - Ma Yaxi Y