CIDEC Polyklonaler Antikörper

CIDEC Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12287-1-AP

Synonyme

Cell death activator CIDE 3, CIDE 3, CIDEC, Fsp27

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 12287-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12287-1-AP (CIDEC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12287-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12287-1-AP (CIDEC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

12287-1-AP bindet in WB, ELISA CIDEC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CIDEC fusion protein Ag2913
• Vollständiger Name: cell death-inducing DFFA-like effector c
• Berechnetes Molekulargewicht: 238 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27-35 kDa
• GenBank-Zugangsnummer: BC016851
• Gene symbol: CIDEC
• Gene ID (NCBI): 63924
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cell death-inducing DFF45-like effector (CIDE) proteins, including CIDEA, CIDEB and CIDEC (also called Fsp27 in rodents), are predominantly expressed in brown adipose tissues (BAT), liver and WAT. CIDEC was found to be highly expressed in adipose tissues and to function as a lipid droplet-binding protein that promotes lipid accumulation in adipocytes. While normal mouse livers express low levels of CIDEC protein, its expression is highly upregulated in NALFD, but not in chronic ethanol feeding-induced fatty liver. (PMID: 26099526) Catalog#12287-1-AP recognizing CIDEC (calculated 28 kDa) as a 30 kDa and 45-50 kDa due to its posttranslational modification (PMID: 19258494).

Publikationen

Species	Application	Title
mouse	WB	J Nutr Biochem; Identification of potential pathogenic hepatic super-enhancers regulatory network in high-fat diet induced hyperlipidemia; Authors - Yingying Hu
human	WB	Commun Biol; Glutathione S-transferase Mu 2 inhibits hepatic steatosis via ASK1 suppression.; Authors - Yi Jin
human	WB	Cancer Biol Ther; The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer; Authors - Wen-Jing Yun
human	WB	Biomed Pharmacother; Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway.; Authors - Xingran Chen
mouse	WB	Aging Cell; Long-term NAD+ supplementation prevents the progression of age-related hearing loss in mice; Authors - Mustafa N Okur
mouse	WB	Cell Death Discov; Shift work promotes adipogenesis via cortisol-dependent downregulation of EGR3-HDAC6 pathway; Authors - Xinxing Wan