CIB1 Polyklonaler Antikörper

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• KD/KO Validated

CIB1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 11823-1-AP

Synonyme

DNA PKcs interacting protein, CIBP, CIB, Calmyrin, Calcium and integrin-binding protein 1

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Galerie der Validierungsdaten

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  WB analysis of human liver using 11823-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11823-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 11823-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11823-1-AP

  IP result of anti-CIB1 (IP:11823-1-AP, 4ug; Detection:11823-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human breast cancer using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 11823-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CIB1 antibody (11823-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, HepG2-Zellen, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

11823-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CIB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CIB1 fusion protein Ag2391
• Vollständiger Name: calcium and integrin binding 1 (calmyrin)
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC000846
• Gene symbol: CIB1
• Gene ID (NCBI): 10519
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3.

Publikationen

Species	Application	Title
human	IP	Cell Death Differ; CHIP-mediated CIB1 ubiquitination regulated epithelial-mesenchymal transition and tumor metastasis in lung adenocarcinoma.; Authors - Yuanqi Liu
human	WB,IF	PLoS Pathog; CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells.; Authors - Chirosree Bandyopadhyay ; KD Validated
mouse	WB	FASEB J; Identification of calcium and integrin-binding protein 1 as a novel regulator of production of amyloid β peptide using CRISPR/Cas9-based screening system.; Authors - Yung Wen Chiu ; KO Validated
rat	WB	Sci Rep; PDI regulates seizure activity via NMDA receptor redox in rats.; Authors - Ji Yang Kim
human	WB, IF	J Virol; p130Cas Scaffolds the Signalosome to Direct Adaptor-Effector Crosstalk During Kaposi's Sarcoma-Associated Herpesvirus Trafficking in Human Microvascular Dermal Endothelial Cells.; Authors - Chirosree Bandyopadhyay ; KD Validated
mouse	WB	Front Mol Neurosci; Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington's disease.; Authors - Magdalena Czeredys