CIAO1 Polyklonaler Antikörper

CIAO1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10295-1-AP

Synonyme

WDR39, WD repeat-containing protein 39, Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 10295-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10295-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10295-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 10295-1-AP

  Immunofluorescent analysis of HepG2 cells using 10295-1-AP (CIAO1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10295-1-AP bindet in WB, IHC, IF/ICC, ELISA CIAO1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CIAO1 fusion protein Ag0349
• Vollständiger Name: cytosolic iron-sulfur protein assembly 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 48-55 kDa
• GenBank-Zugangsnummer: BC001395
• Gene symbol: CIAO1
• Gene ID (NCBI): 9391
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CIAO1, also named as CIA1 or WDR39, is a essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. CIAO1 seems to specifically modulate the transactivation activity of WT1 and may function to regulate the physiological functions of WT1 in cell growth and differentiation. It may also play a role in chromosome segregation. The predicted MW of this protein is 38 kDa. 10295-1-AP detected 48-55 kDa band because of phosphorylation. And 10295-1-AP do well in IHC and IF detection.