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CHRNA5 Polyklonaler Antikörper

CHRNA5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13516-1-AP

Synonyme

CHRNA5, LNCR2, NACHRA5

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of Jurkat cells using CHRNA5 Polyclonal antibody (13516-1-AP)

    WB analysis of Jurkat using 13516-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CHRNA5 Polyclonal antibody (13516-1-AP)

    WB analysis of A549 using 13516-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse stomach tissue using CHRNA5 Polyclonal antibody (13516-1-AP)

    WB analysis of mouse stomach using 13516-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse bladder tissue using CHRNA5 Polyclonal antibody (13516-1-AP)

    WB analysis of mouse bladder using 13516-1-AP

    mouse bladder tissue were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CHRNA5 Polyclonal antibody (13516-1-AP)

    WB analysis of A431 using 13516-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBJurkat-Zellen, A431-Zellen, A549-Zellen, Maus-Blasengewebe, Maus-Magengewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13516-1-AP bindet in WB, ELISA CHRNA5 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CHRNA5 fusion protein Ag4447
Vollständiger Name cholinergic receptor, nicotinic, alpha 5
Berechnetes Molekulargewicht 468 aa, 53 kDa
Beobachtetes Molekulargewicht 53 kDa
GenBank-ZugangsnummerBC033639
Gene symbol CHRNA5
Gene ID (NCBI) 1138
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.

Protokolle

Produktspezifische Protokolle
WB protocol for CHRNA5 antibody 13516-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen