CHRNA1 Polyklonaler Antikörper

CHRNA1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10613-1-AP

Synonyme

CHNRA, ACHRA

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 10613-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10613-1-AP (CHRNA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 10613-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 10613-1-AP (CHRNA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10613-1-AP (CHRNA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10613-1-AP (CHRNA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10613-1-AP (CHRNA1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10613-1-AP (CHRNA1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10613-1-AP (CHRNA1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10613-1-AP (CHRNA1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of C2C12 using 10613-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed C2C12 cell using 10613-1-AP (CHRNA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: C2C12 cell

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 3 publications below

Produktinformation

10613-1-AP bindet in WB, IHC, IF/ICC, ELISA CHRNA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHRNA1 fusion protein Ag0933
• Vollständiger Name: cholinergic receptor, nicotinic, alpha 1 (muscle)
• Berechnetes Molekulargewicht: 54.5 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC006314
• Gene symbol: CHRNA1
• Gene ID (NCBI): 1134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo
human	WB	Sci Rep; Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery.; Authors - Krzysztof M Bernadzki
rat	WB	Front Pharmacol; Miya Improves Osteoarthritis Characteristics via the Gut-Muscle-Joint Axis According to Multi-Omics Analyses.; Authors - Tianyang Xu
mouse	WB	Sci Rep; An improved method for culturing myotubes on laminins for the robust clustering of postsynaptic machinery.; Authors - Marcin Pęziński

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