CHI3L1/YKL40 Polyklonaler Antikörper

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• KD/KO Validated

CHI3L1/YKL40 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 12036-1-AP

Synonyme

CHI3L1, GP 39, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39

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Galerie der Validierungsdaten

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  WB analysis of THP-1 using 12036-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 12036-1-AP (CHI3L1/YKL40 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 12036-1-AP

  WB result of CHI3L1/YKL40 antibody (12036-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and CHI3L1/YKL40 knockout THP-1 cells.

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  IHC staining of human liver using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12036-1-AP (CHI3L1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12036-1-AP (CHI3L1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12036-1-AP (CHI3L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12036-1-AP (CHI3L1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 12036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHI3L1/YKL40 antibody (12036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 12036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHI3L1/YKL40 antibody (12036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 9 publications below
• IF: See 7 publications below

Produktinformation

12036-1-AP bindet in WB, IHC, IF-P, ELISA CHI3L1/YKL40 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHI3L1/YKL40 fusion protein Ag2710
• Vollständiger Name: chitinase 3-like 1 (cartilage glycoprotein-39)
• Berechnetes Molekulargewicht: 383 aa, 43 kDa
• Beobachtetes Molekulargewicht: 40, 33 kDa
• GenBank-Zugangsnummer: BC008568
• Gene symbol: CHI3L1
• Gene ID (NCBI): 1116
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

Publikationen

Species	Application	Title
mouse	IF	Sci Bull (Beijing); Single-cell profile reveals the landscape of cardiac immunity and identifies a cardio-protective Ym-1hi neutrophil in myocardial ischemia-reperfusion injury; Authors - Yalan Dong
human	WB	J Neurol Neurosurg Psychiatry; Molecules of senescent glial cells differentiate Alzheimer's disease from ageing; Authors - Linbin Dai
mouse	IHC, IF, WB	Elife; Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment.; Authors - Zhao Shan
human	IHC	Genomics; MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.; Authors - Yuan Shi
human	IF	Glia; SSEA-4 and YKL-40 positive progenitor subtypes in the subventricular zone of developing human neocortex.; Authors - Christian B Brøchner
human	IHC	Front Oncol; BYSL Promotes Glioblastoma Cell Migration, Invasion, and Mesenchymal Transition Through the GSK-3β/β-Catenin Signaling Pathway.; Authors - Zhuang Sha