CHEK2 Polyklonaler Antikörper

CHEK2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Rezensionen (1)

Kat-Nr. : 13954-1-AP

Synonyme

CHK2, Hucds1, hCds1, EC:2.7.11.1, Cds1 homolog

---

Galerie der Validierungsdaten

• 

  WB analysis of HL-60 using 13954-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13954-1-AP (Chk2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13954-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13954-1-AP (Chk2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13954-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13954-1-AP (Chk2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13954-1-AP

  IP result of anti-CHEK2 (IP:13954-1-AP, 4ug; Detection:13954-1-AP 1:4000) with HeLa cells lysate 1320 ug.

• 

  IF Staining of HepG2 using 13954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHEK2 antibody (13954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phallodin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 29 publications below
• IHC: See 1 publications below

Produktinformation

13954-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CHEK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHEK2 fusion protein Ag5065
• Vollständiger Name: CHK2 checkpoint homolog (S. pombe)
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC004207
• Gene symbol: CHEK2
• Gene ID (NCBI): 11200
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chk2(Checkpoint kinase 2) is also named as CDS1, CHEK2, RAD53 and belongs to the CHK2 subfamily. It is a protein kinase that is activated in response to DNA damage and involved in cell cycle arrest. It also mediates the induction of senescence in fibroblasts, but is dispensable for the induction of telomere dysfunction checkpoints at the stem and progenitor cell level(PMID:20577265). This protein can exsit as a dimer (PMID:19782031). Active forms of CHEK2 devoid of phosphorylation at Thr-68 can form unstable dimers(PMID:12386164). Defects in CHEK2 is a cause of susceptibility to breast cancer (BC)(PMID:21618645). It has 12 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Nat Commun; G-quadruplex DNA structures in human stem cells and differentiation.; Authors - Katherine G Zyner
human	WB	Oncogene; TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex; Authors - Ya Wang
human	WB	Clin Transl Med; Single-cell profiling-guided combination therapy of c-Fos and histone deacetylase inhibitors in diffuse large B-cell lymphoma.; Authors - Luqiao Wang
rat	WB	Int J Nanomedicine; TiO2 Nanoparticles Caused DNA Damage in Lung and Extra-Pulmonary Organs Through ROS-Activated FOXO3a Signaling Pathway After Intratracheal Administration in Rats.; Authors - Bin Han
rat	WB	Ecotoxicol Environ Saf; Hexavalent chromium caused DNA damage repair and apoptosis via the PI3K/AKT/FOXO1 pathway triggered by oxidative stress in the lung of rat; Authors - Lixia Zhang
human	WB	Oncotarget; UBE2D3 gene overexpression increases radiosensitivity of EC109 esophageal cancer cells in vitro and in vivo.; Authors - Xiaojia Gao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Priya (Verified Customer) (01-17-2023)

I have used this antibody for human keratinocytes, cardiomyocytes, mouse skin and liver tissues

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, mouse Liver and skin