CGGBP1 Polyklonaler Antikörper

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• KD/KO Validated

CGGBP1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, chIP, ELISA, Dot Blot

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10716-1-AP

Synonyme

20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein

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Galerie der Validierungsdaten

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  WB analysis using 10716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10716-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10716-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10716-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10716-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10716-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10716-1-AP (CGGBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, Maus-Thymusgewebe, Raji-Zellen, Ratten-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

10716-1-AP bindet in WB, IP, IF, IHC, chIP, ELISA, Dot Blot CGGBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CGGBP1 fusion protein Ag1129
• Vollständiger Name: CGG triplet repeat binding protein 1
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19-23 kDa
• GenBank-Zugangsnummer: BC005222
• Gene symbol: CGGBP1
• Gene ID (NCBI): 8545
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)

Publikationen

Species	Application	Title
human	IP	Cell Cycle; CGGBP1 phosphorylation constitutes a telomere-protection signal.; Authors - Umashankar Singh ; KD Validated
human	chIP	Cell Cycle; Growth signals employ CGGBP1 to suppress transcription of Alu-SINEs.; Authors - Prasoon Agarwal
human	WB,IP	Epigenetics Chromatin; CGGBP1 regulates CTCF occupancy at repeats.; Authors - Divyesh Patel
human	IF	Exp Cell Res; CGGBP1 is a nuclear and midbody protein regulating abscission.; Authors - Singh Umashankar U
human	WB,Dot Blot	BMC Cancer; An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1.; Authors - Manthan Patel
human	WB	BMC Genet; CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity.; Authors - Manthan Patel