Cofilin 2 Polyklonaler Antikörper

Cofilin 2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11848-1-AP

Synonyme

CFL2, CFL 2, NEM7, Cofilin-2, Cofilin2

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Galerie der Validierungsdaten

• 

  WB analysis of human liver using 11848-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11848-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11848-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11848-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11848-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11848-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11848-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human normal colon using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human gliomas using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of L02 using 11848-1-AP

  Immunofluorescent analysis of L02 cells using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, L02-Zellen, Mauslebergewebe, Mauslungengewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: human normal colon, humanes Gliomgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11848-1-AP bindet in WB, IHC, IF/ICC, ELISA Cofilin 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cofilin 2 fusion protein Ag2408
• Vollständiger Name: cofilin 2 (muscle)
• Berechnetes Molekulargewicht: 166 aa, 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC022876
• Gene symbol: Cofilin 2
• Gene ID (NCBI): 1073
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Mol Ther; Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells.; Authors - Lara Ottaviani