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Complement factor B Monoklonaler Antikörper

Complement factor B Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

8D2B6

Kat-Nr. : 66154-1-Ig

Synonyme

BF, BFD, C3/C5 convertase, CFAB, CFB, Complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of human plasma using Complement factor B Monoclonal antibody (66154-1-Ig)

    WB analysis of human plasma using 66154-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66154-1-Ig (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IHC staining of human liver using 66154-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IHC staining of human liver using 66154-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IF Staining of human liver using 66154-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IF Staining of human liver using 66154-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Blutgewebe
Erfolgreiche Detektion in IHChumanes Lebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFhumanes Lebergewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66154-1-Ig bindet in WB, IHC, IF, ELISA Complement factor B und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgM
Klonalität Monoklonal
Typ Antikörper
Immunogen Complement factor B fusion protein Ag19107
Vollständiger Name complement factor B
Berechnetes Molekulargewicht 86 kDa
Beobachtetes Molekulargewicht 93 kDa
GenBank-ZugangsnummerBC007990
Gene symbol CFB
Gene ID (NCBI) 629
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Thiophiler Affinitätschromatograph
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

Protokolle

Produktspezifische Protokolle
WB protocol for Complement factor B antibody 66154-1-IgProtokoll herunterladen
IHC protocol for Complement factor B antibody 66154-1-IgProtokoll herunterladen
IF protocol for Complement factor B antibody 66154-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen