CETP Polyklonaler Antikörper

CETP Polyklonal Antikörper für WB, IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13459-1-AP

Synonyme

CETP, HDLCQ10, Lipid transfer protein I

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Galerie der Validierungsdaten

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  WB analysis of human brain using 13459-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13459-1-AP (CETP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 13459-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CETP antibody (13459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13459-1-AP bindet in WB, IF, IHC, ELISA CETP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CETP fusion protein Ag3923
• Vollständiger Name: cholesteryl ester transfer protein, plasma
• Berechnetes Molekulargewicht: 493 aa, 55 kDa
• Beobachtetes Molekulargewicht: 50-70, 100 kDa
• GenBank-Zugangsnummer: BC025739
• Gene symbol: CETP
• Gene ID (NCBI): 1071
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cholesteryl ester transfer protein (CETP) is a plasma glycoprotein which transfers cholesteryl esters, triglycerides, and phospholipids between lipoproteins. In plasma, CETP is mainly associated with HDL particles and catalyzes the transfer of cholesteryl esters from HDL to apolipoprotein (apo) B-containing plasma lipoproteins. Low plasma concentrations of high-density lipoprotein-cholesterol (HDL-C) have long been recognized as a major, independent cardiovascular disease (CVD) risk factor. It demonstrated that altered level of plasma CETP was associated with the increased risk of CVD (PMID: 17991755, PMID: 23477743). CETP can be detected a 70 kDa isoform by glycosylation (PMID: 3281933,PMID: 22056562). It also can be detected 100 kDa dimer (PMID: 7744792).

Publikationen

Species	Application	Title
human	WB, IF	J Biol Chem; Regulation of hepatic cholesteryl ester transfer protein expression and reverse cholesterol transport by inhibition of DNA topoisomerase II.; Authors - Mengyang Liu
human	IHC	J Transl Med; Characterization of the proteome of stable and unstable carotid atherosclerotic plaques using data-independent acquisition mass spectrometry; Authors - Zhichao Lai