CEBPA Polyklonaler Antikörper

CEBPA Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29388-1-AP

Synonyme

C/EBP alpha, CEBP, CEBPA

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Galerie der Validierungsdaten

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  WB analysis using 29388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29388-1-AP (CEBPA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29388-1-AP (CEBPA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of THP-1 using 29388-1-AP

  IP result of anti-CEBPA(IP:29388-1-AP, 4ug; Detection:29388-1-AP 1:4000) with THP-1 cells lysate 1520 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BGC-823-Zellen, HL-60-Zellen, K-562-Zellen, L02-Zellen, LNCaP-Zellen, THP-1-Zellen, U-937-Zellen
• Erfolgreiche IP: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29388-1-AP bindet in WB, IP, ELISA CEBPA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEBPA fusion protein Ag29947
• Vollständiger Name: CCAAT/enhancer binding protein (C/EBP), alpha
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: NM_004364
• Gene symbol: CEBPA
• Gene ID (NCBI): 1050
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc. The calcualted molecular weight of CEBPA is 38 kDa, but modified CEBPA is about 42 kDa (PMID: 19623175).