CDSN Polyklonaler Antikörper

CDSN Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13184-1-AP

Synonyme

CDSN, corneodesmosin, D6S586E, HTSS

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Galerie der Validierungsdaten

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  WB analysis using 13184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13184-1-AP (CDSN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 13184-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13184-1-AP (CDSN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13184-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13184-1-AP (CDSN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 13184-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13184-1-AP (CDSN antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 13184-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13184-1-AP (CDSN antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

13184-1-AP bindet in WB, IF, IHC, ELISA CDSN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDSN fusion protein Ag3854
• Vollständiger Name: corneodesmosin
• Berechnetes Molekulargewicht: 528 aa, 52 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC031993
• Gene symbol: CDSN
• Gene ID (NCBI): 1041
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDSN, also named as S protein, is important for the epidermal barrier integrity. CDSN is a unique extracellular 52- to 56-kDa glycoprotein found in corneodesmosomes. Pathogenic mutations in CDSN have been linked in humans to genodermatoses affecting both the hair follicle and the epidermis. Inflammatory peeling skin syndrome is caused a novel mutation in CDSN.

Publikationen

Species	Application	Title
mouse	WB	J Invest Dermatol; Sphingosine 1-Phosphate Receptor 2 Is Central to Maintaining Epidermal Barrier Homeostasis.; Authors - Satomi Igawa
human	IF	Exp Dermatol; Acral peeling in Nagashima type palmo-plantar keratosis patients reveals the role of serine protease inhibitor B 7 in keratinocyte adhesion.; Authors - Eran Cohen-Barak
human	IHC	Clin Cosmet Investig Dermatol; Repeated short climatic change affects the epidermal differentiation program and leads to matrix remodeling in a human organotypic skin model.; Authors - Laetitia-Barbollat Boutrand
human	IHC	Arch Dermatol Res; Inflammatory peeling skin syndrome caused a novel mutation in CDSN.; Authors - Telem Dana Fuchs DF