CDR2 Polyklonaler Antikörper

CDR2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (2)

Kat-Nr. : 11611-2-AP

Synonyme

CDR2, CDR62, PCD17, Yo

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Galerie der Validierungsdaten

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  WB analysis of human brain using 11611-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11611-2-AP (CDR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11611-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11611-2-AP (CDR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 11611-2-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11611-2-AP (CDR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11611-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11611-2-AP (CDR2 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

11611-2-AP bindet in WB, IP, IHC, ELISA CDR2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDR2 fusion protein Ag2183
• Vollständiger Name: cerebellar degeneration-related protein 2, 62kDa
• Berechnetes Molekulargewicht: 454 aa, 52 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC017503
• Gene symbol: CDR2
• Gene ID (NCBI): 1039
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Patients with paraneoplastic cerebellar degeneration (PCD) carry a characteristic antibody called anti-Yo. On Western blot analysis of Purkinje cells and tumor tissue from CD patient, the anti-Yo sera react with at least 2 antigens, a major species of 62 kD called CDR62 or CDR2 [PMID:18045792]. CDR2 is partly characterized, as CDR2 through its leucine zipper motif has been demonstrated to interact with c-myc, with cell cycle-related proteins and with a protein kinase, indicating that CDR2 is involved in signal transduction and gene transcription. Furthermore, CDR2 has been found to attenuate hypoxic response in renal cell carcinoma, and recent data suggest a role for CDR2 and c-myc in mitosis in cycling cells [PMID:21080165].

Publikationen

Species	Application	Title
human	IP, WB	Acta Neuropathol; Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis.; Authors - Manja Schubert

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (11-11-2019)

CDR2 was successfully IP'ed from A549 cells. The antibody had not background bands.

• Applications: Immunoprecipitation,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: A594 cells

FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size and no additional background bands were observed around the size range. Perfect!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T, U2OS, HaCaT

CDR2 Antibody Western Blot, validation (1:1000 dilution) in 293T, U2OS, HaCaT (Cat no:11611-2-AP)