VE-cadherin Polyklonaler Antikörper

VE-cadherin Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27956-1-AP

Synonyme

7B4, 7B4 antigen, Cadherin 5, CD144, CDH5, FLJ17376, Vascular endothelial cadherin, VE cadherin



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHUVEC-Zellen, humanes Plazenta-Gewebe
Erfolgreiche Detektion in IHChumanes Plazenta-Gewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in FCHUVEC-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:100-1:400
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 5 publications below
IFSee 5 publications below

Produktinformation

27956-1-AP bindet in WB, IHC, IF, FC, ELISA VE-cadherin und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen VE-cadherin fusion protein Ag27487
Vollständiger Name cadherin 5, type 2 (vascular endothelium)
Berechnetes Molekulargewicht 88 kDa
Beobachtetes Molekulargewicht120-140 kDa
GenBank-ZugangsnummerNM_001795
Gene symbol CDH5
Gene ID (NCBI) 1003
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

Protokolle

Produktspezifische Protokolle
WB protocol for VE-cadherin antibody 27956-1-APProtokoll herunterladen
IHC protocol for VE-cadherin antibody 27956-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB

Nat Commun

FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment

Authors - Zongchao Sun
humanIF

Bioengineering (Basel)

Organotypic 3D Co-Culture of Human Pleura as a Novel In Vitro Model of Staphylococcus aureus Infection and Biofilm Development

Authors - Olga Kurow
WB

Front Cell Infect Microbiol

Analysis of miRNAs Involved in Mouse Heart Injury Upon Coxsackievirus A2 Infection.

Authors - Zhaoke Wu
humanIF

Bioengineering (Basel)

A Transwell-Based Vascularized Model to Investigate the Effect of Interstitial Flow on Vasculogenesis

Authors - Pengwei Deng
humanIF

J Hypertens

Short-chain acyl-CoA dehydrogenase is a potential target for the treatment of vascular remodelling

Authors - Xiaoyi Zhong
IF

Oral Dis

Artemisinin suppressed tumour growth and induced vascular normalisation in oral squamous cell carcinoma via inhibition of MIF

Authors - Xiang-Hua Yu