Cadherin-13 Polyklonaler Antikörper

Cadherin-13 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12618-1-AP

Synonyme

Cadherin 13, Cadherin-13, CDH13, CDHH, H cadherin, H-cadherin, Heart cadherin, P105, T cad, T cadherin, T-cadherin, Truncated cadherin

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse heart using 12618-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12618-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12618-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12618-1-AP

  IP result of anti-Cadherin-13 (IP:12618-1-AP, 4ug; Detection:12618-1-AP 1:700) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human endometrial cancer using 12618-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12618-1-AP (Cadherin-13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 12618-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12618-1-AP (Cadherin-13 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12618-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12618-1-AP (Cadherin-13 antibody at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, L02-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

12618-1-AP bindet in WB, IHC, IP, ELISA, IF Cadherin-13 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cadherin-13 fusion protein Ag3295
• Vollständiger Name: cadherin 13, H-cadherin (heart)
• Berechnetes Molekulargewicht: 713 aa, 78 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC030653
• Gene symbol: Cadherin-13
• Gene ID (NCBI): 1012
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-13 (CDH13, also known as H-cadherin or T-cadherin) is an atypical member of cadherin family, lacking transmembrane and cytosolic domains and anchored to the exterior surface of the plasma membrane via a GPI anchor. CDH13 is thought to affect cellular behavior largely through its signaling properties. It is highly expressed in heart, and in the CNS, CDH13 is expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. It is often down-regulated in cancerous cells. The CDH13 cDNA encodes a 713-amino acid protein, which has a calculated molecular weight of 78 kDa. Studies have detected variable bands on Western blots, including 105 kDa (mature form), 130 kDa (precursor), and 45 kDa, which may be a post-translational processing variant of CDH13 (PMID: 19399994).

Publikationen

Species	Application	Title
mouse	IF	Cell Rep; Macrophages preserve endothelial cell specialization in the adrenal gland to modulate aldosterone secretion and blood pressure; Authors - Zheng Fan

Verwandte Produkte

E-cadherin Polyclonal antibody

20874-1-AP

TGF Beta 1 Polyclonal antibody

18978-1-AP

N-cadherin Monoclonal antibody

66219-1-Ig

Adiponectin Monoclonal antibody

66239-1-Ig