CDCA8 Polyklonaler Antikörper

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• KD/KO Validated

CDCA8 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12465-1-AP

Synonyme

hDasra B, Dasra-B, Dasra B, Cell division cycle-associated protein 8, Borealin

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 12465-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12465-1-AP (CDCA8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12465-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12465-1-AP (CDCA8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 12465-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12465-1-AP (CDCA8 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 12465-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12465-1-AP (CDCA8 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 12465-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12465-1-AP (CDCA8 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

12465-1-AP bindet in WB, IHC, IF/ICC, ELISA CDCA8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDCA8 fusion protein Ag3136
• Vollständiger Name: cell division cycle associated 8
• Berechnetes Molekulargewicht: 280 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31-38 kDa
• GenBank-Zugangsnummer: BC016944
• Gene symbol: CDCA8
• Gene ID (NCBI): 55143
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.

Publikationen

Species	Application	Title
human	IF	Science; ESCRT-III governs the Aurora B-mediated abscission checkpoint through CHMP4C.; Authors - Jeremy G Carlton
human	IHC	Front Pharmacol; A novel risk model of three SUMOylation genes based on RNA expression for potential prognosis and treatment sensitivity prediction in kidney cancer; Authors - Song-Chao Li
human	WB,IHC	J Cell Mol Med; Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma; Authors - Xiao-Han Cui
mouse	WB	Cell Mol Life Sci; Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse; Authors - Haojie Li
human	WB,IHC	Funct Integr Genomics; Let-7c-5p down-regulates immune-related CDCA8 to inhibit hepatocellular carcinoma; Authors - Wanjin Chen
human	IHC,WB	Am J Transl Res; Cell division cycle associated 8 promotes the growth and inhibits the apoptosis of endometrial cancer cells by regulating cell cycle and P53/Rb signaling pathway; Authors - Wenxue Li ; KD Validated