CDC6 Monoklonaler Antikörper

CDC6 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

6F12D2

Kat-Nr. : 66021-1-Ig

Synonyme

Cdc18 related protein, CDC18L, CDC6, CDC6 related protein, HsCDC18, HsCDC6, p62(cdc6)

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Galerie der Validierungsdaten

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  WB analysis using 66021-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66021-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66021-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66021-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66021-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 66021-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66021-1-Ig (CDC6 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, NIH/3T3-Zellen, Raji-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66021-1-Ig bindet in WB, IF, ELISA CDC6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CDC6 fusion protein Ag17958
• Vollständiger Name: cell division cycle 6 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 560 aa, 63 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC025232
• Gene symbol: CDC6
• Gene ID (NCBI): 990
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human CDC6.