CDC34 Polyklonaler Antikörper

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• KD/KO Validated

CDC34 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10964-2-AP

Synonyme

CDC34, E2 CDC34, UBC3, UBCH3, UBE2R1, Ubiquitin protein ligase R1

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Galerie der Validierungsdaten

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  WB analysis of human pancreas using 10964-2-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 10964-2-AP (CDC34 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10964-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10964-2-AP (CDC34 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 10964-2-AP

  IP result of anti-CDC34 (IP:10964-2-AP, 4ug; Detection:10964-2-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IP experiment of HEK-293 using 10964-2-AP

  IP result of anti-CDC34 (IP:10964-2-AP, 4ug; Detection:10964-2-AP 1:300) with HEK-293 cells lysate 2000 ug.

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  IHC staining of human prostate cancer using 10964-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10964-2-AP (CDC34 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Pankreasgewebe, humanes Hirngewebe
• Erfolgreiche IP: Maushodengewebe, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

10964-2-AP bindet in WB, IP, IHC, ELISA CDC34 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDC34 fusion protein Ag1438
• Vollständiger Name: cell division cycle 34 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC009850
• Gene symbol: CDC34
• Gene ID (NCBI): 997
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC34 is also named as UBCH3, UBE2R1(Ubiquitin-conjugating enzyme E2 R1) and belongs to the ubiquitin-conjugating enzyme family. Cdc34 enzyme is itself a substrate for the type of polyubiquitin modification that is known to cause targeting of proteins for degradation. It is phosphorylated in vivo, providing yet another potential means for control of its activity. And the predominantly nuclear localization of this enzyme suggests that substrate molecules relevant to its cell cycle function are similarly located within the nucleus(PMID:8164658). Human CDC34 could substitute efficiently for yeast CDC34(PMID:8248134). It can form a homodimer(PMID:8383676). This antibody can also recognize CDC34B(UBC3B) which is highly homologous to UBC3 (CDC34) with only 47 amino acid differences.

Publikationen

Species	Application	Title
human	WB	J Cell Sci; A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.; Authors - Fabienne C Fiesel
mouse	WB	Cell Rep; Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.; Authors - Stephen Gray
human	WB	Biochem Biophys Rep; A siRNA screening of UBE2 family demonstrated that UBE2R1 had a high repressive effect on HIV Tat protein; Authors - Muyu Xu ; KD Validated