CDK1 Polyklonaler Antikörper

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• KD/KO Validated

CDK1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(241)

Kat-Nr. : 19532-1-AP

Synonyme

CDK1-Specific, CDC2, CDC28A, CDKN1, Cell division protein kinase 1

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  WB analysis of HeLa using 19532-1-AP

  WB result of CDK1-Specific antibody (19532-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK1-Specific transfected HeLa cells.

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  WB analysis using 19532-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19532-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19532-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19532-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19532-1-AP

  IP result of anti-CDK1-Specific (IP:19532-1-AP, 4ug; Detection:19532-1-AP 1:5000) with HeLa cells lysate 1600 ug.

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  IP experiment of HEK-293 using 19532-1-AP

  IP result of anti-CDK1-Specific (IP:19532-1-AP, 4ug; Detection:19532-1-AP 1:5000) with HEK-293 cells lysate 1200 ug.

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  IHC staining of human cervical cancer using 19532-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP (CDK1-Specific antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 19532-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP (CDK1-Specific antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of rat liver using 19532-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 19532-1-AP (CDK1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 19532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19532-1-AP (CDK1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19532-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK1-Specific antibody (19532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 19532-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK1-Specific (19532-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, C2C12-Zellen, C6-Zellen, COS-7-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, Mauslebergewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 219 publications below
• IHC: See 24 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

19532-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA CDK1 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cell division cycle 2, G1 to S and G2 to M
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: NM_001786
• Gene symbol: CDK1
• Gene ID (NCBI): 983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

Publikationen

Species	Application	Title
human	WB	Signal Transduct Target Ther; SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis; Authors - Saisai Guo ; KD Validated
human	WB	Nat Cell Biol; Caspase-2 is a condensate-mediated deubiquitinase in protein quality control; Authors - Yingwei Ge
human	IHC	Cell Res; Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma.; Authors - Junya Peng
human	WB	Nat Struct Mol Biol; Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis; Authors - Wei Zhang
human	WB	Nat Commun; Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells; Authors - Qiuhong Zhu
mouse	WB	Nat Commun; Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regeneration; Authors - Dongcheng Cai