CD82 Polyklonaler Antikörper

CD82 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10248-1-AP

Synonyme

4F9, C33, C33 antigen, CD82 antigen, CD82 molecule, CD82, KAI1, GR15, IA4, Inducible membrane protein R2, KAI1, Metastasis suppressor Kangai 1, R2, SAR2, ST6, Tetraspanin 27, Tspan 27, TSPAN27

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 10248-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10248-1-AP (CD82 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10248-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10248-1-AP (CD82 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Mammakarzinomgewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10248-1-AP bindet in WB, IF, IHC, ELISA CD82 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD82 fusion protein Ag0314
• Vollständiger Name: CD82 molecule
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC000726
• Gene symbol: CD82
• Gene ID (NCBI): 3732
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.

Publikationen

Species	Application	Title
human	IF	Adv Biol (Weinh); Tetraspanin CD82 Associates with Trafficking Vesicle in Muscle Cells and Binds to Dysferlin and Myoferlin; Authors - Tatiana Fontelonga
human	WB	Oncol Lett; MicroRNA-633 enhances melanoma cell proliferation and migration by suppressing KAI1.; Authors - Zhengxiang Wang
human	IHC	Asian Pac J Cancer Prev; Down Regulation of KAI1/CD82 in Lymph Node Positive and Advanced T-Stage Group in Breast Cancer Patients.; Authors - Thammineni Krishna Latha
mouse	WB	Parasit Vectors; Quantitative proteomic analysis of local and systemic extracellular vesicles during Eimeria falciformis infectious cycle in the host; Authors - Joshua Seun Olajide