CD63 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD63 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (7)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(466)

Rezensionen (4)

Kat-Nr. : 25682-1-AP

Synonyme

CD 63, CD63 antigen, Limp1, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1

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  WB analysis using 25682-1-AP

  Human plasma and human plasma-derived exosomes (human plasma-exo) were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human urine exosomes using 25682-1-AP

  human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human serum exosomes using 25682-1-AP

  Human serum exosomes were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human malignant melanoma using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of human tonsillitis using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 25682-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human malignant melanoma using 25682-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (25682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human malignant melanoma using 25682-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (25682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 25682-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD63 antibody (25682-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human urine exosomes tissue, A375-Zellen, humane Serumexosome, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe, humanes Lymphomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes malignes Melanomgewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:40000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 437 publications below
• IHC: See 8 publications below
• IF: See 20 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

25682-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA CD63 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, hamster, Hausschwein, Huhn, Kaninchen, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD63 fusion protein Ag19690
• Vollständiger Name: CD63 molecule
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 30-60 kDa
• GenBank-Zugangsnummer: BC002349
• Gene symbol: CD63
• Gene ID (NCBI): 967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

Publikationen

Species	Application	Title
human	WB	Cell Res; In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.; Authors - Zheng Fu
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
	WB	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
human	WB	Adv Sci (Weinh); Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury; Authors - Xun Wu
human	WB	Adv Sci (Weinh); Tβ4-Engineered ADSC Extracellular Vesicles Rescue Cell Senescence Through Separable Microneedle Patches for Diabetic Wound Healing; Authors - Youjun Ding
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Federica (Verified Customer) (11-30-2023)

I used a Leica Bond Max on a healthy Tonsil tissue. Antigen Retrival ER2 95C for 30min

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Tonsil

CD63 Antibody Immunohistochemistry validation (1:1000 dilution) in Tonsil (Cat no:25682-1-AP)

FH

Nydia (Verified Customer) (06-11-2022)

The HeLa cells as well as Lamp1-expressing stem cells used in the immunostaining were transfected or alternatively transfected by splitting the cells later on the glass coverslips. In order to increase cell spreading and adhesion, the coverslips used were acid washed and treated with fibronectin. Afterwards, the cells were fixed with 4% PFA, permeabilized with 0.2% Triton X-100 in phosphate- buffered saline (PBS) for 10 minutes and for 1 hour the cells were blocked with 5% BSA in PBS. Then overnight, at 4 degrees Celsius, the primary antibodies were added followed by a wash with PBS 3 times.

• Applications: Cell culture
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

CD63 Antibody Cell culture validation (1:1000 dilution) in HeLa cells (Cat no:25682-1-AP)

FH

Guorong (Verified Customer) (03-22-2022)

Smears between 30 and 60 kDa was detected

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

CD63 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:25682-1-AP)

FH

David (Verified Customer) (03-30-2020)

Used for both SH-SY5Y lysate and exosomes/ extracellular vesicles. Very good signal in lysates (could be used at much higher dilution). Also good signal in exosomes, which are notoriously difficult to assess by immunoblot. Very impressive results compared to other markers.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: SH-SY5Y