CD45R (B220) Monoklonaler Antikörper

CD45R (B220) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

human, Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

RA3-6B2

Kat-Nr. : PE-65139

Synonyme

B220, CD45R, Cd45, Ptprc, EC:3.1.3.48

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  FC experiment of C57BL/6 mouse splenocytes using PE-65139

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD45R (B220) (PE-65139, Clone: RA3-6B2) or 0.2 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3), and CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were not fixed.

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  FC experiment of human PBMCs using PE-65139

  1X10^6 human PBMCs were surface stained with 0.5 ug PE Anti-Mouse CD45R (B220) (PE-65139, Clone:RA3-6B2) or Rat IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using PE-65139

  1X10^6 human PBMCs were surface stained with 0.5 ug PE Anti-Mouse CD45R (B220) (PE-65139, Clone:RA3-6B2) or Rat IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten, humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65139 bindet in FC CD45R (B220) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Murines Abelson-Leukämie-virusinduzierte Vorläufer-B-Tumorzellen
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC028512
• Gene symbol: CD45
• Gene ID (NCBI): 19264
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.