CD45 Monoklonaler Antikörper
CD45 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
WB, IHC, IF, FC, ELISA
Konjugation
Unkonjugiert
CloneNo.
4E9B2
Kat-Nr. : 60287-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Hausschwein-Milzgewebe, Raji-Zellen |
| Erfolgreiche Detektion in IHC | humanes Appendizitis-Gewebe, humanes Kolongewebe, humanes Leberkarzinomgewebe, humanes Tonsillitisgewebe, Mausmilzgewebe, Ratten-Kolongewebe, Ratten-Thymusgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | Maus-Kolongewebe, humanes Tonsillitisgewebe, Mausmilzgewebe |
| Erfolgreiche Detektion in FC | Raji-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunhistochemie (IHC) | IHC : 1:4000-1:16000 |
| Immunfluoreszenz (IF) | IF : 1:500-1:2000 |
| Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 1 publications below |
| IHC | See 13 publications below |
| IF | See 17 publications below |
| FC | See 7 publications below |
Produktinformation
60287-1-Ig bindet in WB, IHC, IF, FC, ELISA CD45 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | CD45 fusion protein Ag13712 |
| Vollständiger Name | protein tyrosine phosphatase, receptor type, C |
| Berechnetes Molekulargewicht | 1304 aa, 147 kDa |
| Beobachtetes Molekulargewicht | 220 kDa |
| GenBank-Zugangsnummer | BC014239 |
| Gene symbol | PTPRC |
| Gene ID (NCBI) | 5788 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Protein tyrosine phosphatase receptor type C (PTPRC) is also known as CD45 antigen, which was originally called leukocyte common antigen (LCA). CD45 is an enzyme required for T-cell activation through the antigen receptor consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 via an interaction through the first PTPase domain, which is the only domain to possess intrinsic kinase activity. However, both domains are required for appropriate phosphatase and T-cell antigen receptor activity (PMID: 8076596).
What is the molecular weight of CD45?
The molecular weight of CD45 is a 180-240 kDa.
What are the isoforms of CD45?
Six different human isoforms of CD45 have been isolated, containing all three exons (ABC isoform), two of the three exons (AB and BC isoforms), only one exon (A isoform or B isoform), or no exons (O isoform). All of the isoforms have the same eight amino acids at their amino-terminus (PMID: 12412720).
What is the tissue specificity of CD45?
CD45 is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets (PMID: 7803253).
What are the post-translational modifications of CD45?
CD45 can be heavily N- and O-glycosylated (PMID: 1702721).
What is CD45's involvement in disease?
Defects in CD45 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); a severe form of SCID. It is classed by the impairment of both humoral and cell-mediated immunity, leukopenia, and the low or absent levels of antibodies (PMID: 10700239). Also, genetic variants of CD45 have a role in susceptibility to multiple sclerosis (PMID: 12864992).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for CD45 antibody 60287-1-Ig | Protokoll herunterladen |
| IHC protocol for CD45 antibody 60287-1-Ig | Protokoll herunterladen |
| IF protocol for CD45 antibody 60287-1-Ig | Protokoll herunterladen |
| FC protocol for CD45 antibody 60287-1-Ig | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Commun Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy | ||
Theranostics Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis. | ||
Cell Biol Toxicol Induction of Sestrin2 by pterostilbene suppresses ethanol-triggered hepatocyte senescence by degrading CCN1 via p62-dependent selective autophagy. | ||
Neural Regen Res Transplantation of fibrin-thrombin encapsulated human induced neural stem cells promotes functional recovery of spinal cord injury rats through modulation of the microenvironment | ||
Stem Cell Reports Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation. | ||
Stem Cell Res Ther Hepatic progenitor cells promote the repair of schistosomiasis liver injury by inhibiting IL-33 secretion in mice. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (01-09-2023) | A good Hematopoietic cell marker for IF. Little background observed.
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