CD45.2 Monoklonaler Antikörper

CD45.2 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

104

Kat-Nr. : CL555-65072

Synonyme

B220, Cd45, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200

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Galerie der Validierungsdaten

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  FC experiment of C57BL/6 mouse splenocytes using CL555-65072

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD45.2 (CL555-65072, Clone:104), or 0.5 ug CoraLite® Plus 555 Mouse IgG2a Isotype Control (C1.18.4) (CL555-65208, Clone: C1.18.4). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: C57BL/6 mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-65072 bindet in FC CD45.2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: B10.S-Maus-Thymozyten und -Splenozyten
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC028512
• Gene symbol: Ptprc
• Gene ID (NCBI): 19264
• Konjugation: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).