CD44 Monoklonaler Antikörper

CD44 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

6F4H2

Kat-Nr. : CL594-60224

Synonyme

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

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Galerie der Validierungsdaten

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  IF Staining of human lung cancer using CL594-60224

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®594 CD44 antibody (CL594-60224, Clone: 6F4H2 ) at dilution of 1:200.

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  IF Staining of human lung cancer using CL594-60224

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®594 CD44 antibody (CL594-60224, Clone: 6F4H2 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Lungenkarzinomgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60224 bindet in IF CD44 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD44 fusion protein Ag7633
• Vollständiger Name: CD44 molecule (Indian blood group)
• Berechnetes Molekulargewicht: 742 aa, 82 kDa
• GenBank-Zugangsnummer: BC004372
• Gene symbol: CD44
• Gene ID (NCBI): 960
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.