CD44 Polyklonaler Antikörper

CD44 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30854-1-AP

Synonyme

AU023126, AW121933, AW146109, Cd44, CD44 antigen, HERMES, Ly 24, Pgp 1

---

Galerie der Validierungsdaten

• 

  WB analysis using 30854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30854-1-AP (Cd44 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: J774A.1 cells, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30854-1-AP bindet in WB, ELISA CD44 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD44 fusion protein Ag33515
• Vollständiger Name: CD44 antigen
• Berechnetes Molekulargewicht: 86kd
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: NM_009851
• Gene symbol: Cd44
• Gene ID (NCBI): 12505
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.