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CD300LG Polyklonaler Antikörper

CD300LG Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15426-1-AP

Synonyme

CD300LG, CLM 9, CLM9, CMRF35 like molecule 9, NEPMUCIN, TREM 4, TREM4

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of human kidney tissue using CD300LG Polyclonal antibody (15426-1-AP)

    WB analysis of human kidney using 15426-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15426-1-AP (CD300LG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CD300LG Polyclonal antibody (15426-1-AP)

    WB analysis of HepG2 using 15426-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15426-1-AP (CD300LG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CD300LG Polyclonal antibody (15426-1-AP)

    IF Staining of HEK-293 using 15426-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15426-1-AP (CD300LG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Nierengewebe, HepG2-Zellen
Erfolgreiche Detektion in IFHEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15426-1-AP bindet in WB, IF,ELISA CD300LG und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD300LG fusion protein Ag8075
Vollständiger Name CD300 molecule-like family member g
Berechnetes Molekulargewicht 36 kDa
Beobachtetes Molekulargewicht 36 kDa
GenBank-ZugangsnummerBC025395
Gene symbol CD300LG
Gene ID (NCBI) 146894
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD300LG, also known as CLM9 (CMRF35-like molecule 9), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LG is a receptor which may mediate L-selectin-dependent lymphocyte rollings and may play an important role in inflammation.

Protokolle

Produktspezifische Protokolle
WB protocol for CD300LG antibody 15426-1-APProtokoll herunterladen
IF protocol for CD300LG antibody 15426-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen