CD274 (PD-L1, B7-H1) Monoklonaler Antikörper

CD274 (PD-L1, B7-H1) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b

Getestete Reaktivität

Maus und mehr (1)

Anwendung

FC

Konjugation

PE Fluorescent Dye

Publikationen

1

CloneNo.

10F.9G2

Kat-Nr. : PE-65073

Synonyme

B7 H1, Cd274, CD274 antigen, PD L1, Pdcd1l1, Pdcd1lg1

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using PE-65073

  1X10^6 mouse splenocytes cells were stained with 0.20ug CD274 (PD-L1, B7-H1) antibody (PE-65073, red) and control antibody (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

PE-65073 bindet in FC CD274 (PD-L1, B7-H1) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Ratte / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD274 antigen
• GenBank-Zugangsnummer: BC066841
• Gene symbol: Cd274
• Gene ID (NCBI): 60533
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Publikationen

Species	Application	Title
human	FC	Biochem Pharmacol; Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT; Authors - Xing Chang