CD244 Monoklonaler Antikörper

CD244 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

2B4.69

Kat-Nr. : CL405-65270

Synonyme

2B4, CD244, h2B4, NAIL, NKR2B4, SLAMF4

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using CL405-65270

  1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD56 and 5 ul CoraLite® Plus 405 Anti-Human CD244 (CL405-65270, Clone: 2B4.69), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL405-65270

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD244 (CL405-65270, Clone: 2B4.69), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL405-65270 bindet in FC CD244 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD244 molecule, natural killer cell receptor 2B4
• GenBank-Zugangsnummer: BC028073
• Gene symbol: CD244
• Gene ID (NCBI): 51744
• Konjugation: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).