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CD22 Polyklonaler Antikörper

CD22 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21894-1-AP

Synonyme

B cell receptor CD22, BL CAM, CD22, CD22 molecule, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using CD22 Polyclonal antibody (21894-1-AP)

    WB analysis using 21894-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21894-1-AP (CD22 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD22 Polyclonal antibody (21894-1-AP)

    IHC staining of human tonsillitis using 21894-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21894-1-AP (CD22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD22 Polyclonal antibody (21894-1-AP)

    IHC staining of human tonsillitis using 21894-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21894-1-AP (CD22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBDaudi-Zellen, Ramos-Zellen
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IHCSee 1 publications below

Produktinformation

21894-1-AP bindet in WB, IHC,ELISA CD22 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD22 fusion protein Ag16607
Vollständiger Name CD22 molecule
Berechnetes Molekulargewicht 847 aa, 95 kDa
Beobachtetes Molekulargewicht 120-140 kDa
GenBank-ZugangsnummerBC109306
Gene symbol CD22
Gene ID (NCBI) 933
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

Protokolle

Produktspezifische Protokolle
WB protocol for CD22 antibody 21894-1-APProtokoll herunterladen
IHC protocol for CD22 antibody 21894-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

Oncol Lett

Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma.

Authors - Bingsheng Yang
View Article