CD146/MCAM Monoklonaler Antikörper

CD146/MCAM Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

4D8A9

Rezensionen (1)

Kat-Nr. : 66153-1-Ig

Synonyme

CD146, MCAM, MUC18

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 66153-1-Ig

  Untreated and PNGase F-treated lysates of HepG2 cells and L02 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of A375 using 66153-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66153-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66153-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66153-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66153-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66153-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66153-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 66153-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66153-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HUVEC using 66153-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66153-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug CD146/MCAM antibody (66153-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A375-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, HUVEC-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Leberkarzinomgewebe, HUVEC-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• FC: See 1 publications below

Produktinformation

66153-1-Ig bindet in WB, IHC, IF, FC, ELISA CD146/MCAM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD146/MCAM fusion protein Ag11855
• Vollständiger Name: melanoma cell adhesion molecule
• Berechnetes Molekulargewicht: 646 aa, 72 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC056418
• Gene symbol: MCAM
• Gene ID (NCBI): 4162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.

Publikationen

Species	Application	Title
human	FC	Exp Cell Res; Nrf2 activation is involved in osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch.; Authors - Xun Xi
human	WB	Cell Signal; Glioma-derived small extracellular vesicles induce pericyte-phenotype transition of glioma stem cells under hypoxic conditions; Authors - Yue Cheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (02-05-2021)

Not sure whether it works, because I cannot detect anything in whole RPE1 cell lysates.

• Applications: Western Blot

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