CD11c Monoklonaler Antikörper

CD11c Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / Mouse IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

BU15

Kat-Nr. : CL488-65196

Synonyme

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

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Galerie der Validierungsdaten

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  FC experiment of Human peripheral blood granulocytes using CL488-65196

  1X10^6 Human peripheral blood granulocytes were surface stained with 5 ul CoraLite® Plus 488-conjugated Anti-Human CD11c (CL488-65196, Clone: BU15) (green) or isotype control antibody (black). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Granulozyten aus peripherem Blut

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65196 bindet in FC CD11c und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / Mouse IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane dendritische Zellen aus Synovialflüssigkeit
• Vollständiger Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Berechnetes Molekulargewicht: 1169 aa, 129 kDa
• GenBank-Zugangsnummer: BC038237
• Gene symbol: ITGAX
• Gene ID (NCBI): 3687
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).