CCT8 Polyklonaler Antikörper

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• KD/KO Validated

CCT8 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (1)

Kat-Nr. : 12263-1-AP

Synonyme

C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta

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Galerie der Validierungsdaten

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  WB analysis using 12263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12263-1-AP (CCT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12263-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12263-1-AP (CCT8 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12263-1-AP

  WB result of CCT8 antibody (12263-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT8 transfected HEK-293 cells.

• 

  IP experiment of Jurkat using 12263-1-AP

  IP result of anti-CCT8 (IP:12263-1-AP, 4ug; Detection:12263-1-AP 1:500) with Jurkat cells lysate 3160 ug.

• 

  IF Staining of SW480 using 12263-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SW480 cells using CCT8 antibody (12263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 12263-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CCT8 antibody 12263-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Mausnierengewebe, Raji-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IF: SW480-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

12263-1-AP bindet in WB, IP, IF, ELISA CCT8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCT8 fusion protein Ag2926
• Vollständiger Name: chaperonin containing TCP1, subunit 8 (theta)
• Berechnetes Molekulargewicht: 548 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC012584
• Gene symbol: CCT8
• Gene ID (NCBI): 10694
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.(PMID:20080638)

Publikationen

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita
mouse	WB	Nat Genet; DYX1C1 is required for axonemal dynein assembly and ciliary motility.; Authors - Tarkar Aarti A
mouse	WB	Neuron; Network organization of the huntingtin proteomic interactome in mammalian brain.; Authors - Dyna I Shirasaki
human	WB	Nat Commun; Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan.; Authors - Alireza Noormohammadi
human	WB,IF	Proc Natl Acad Sci U S A; BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.; Authors - Seo Seongjin S
human	WB	PLoS One; TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.; Authors - Somalinga Balajee R BR

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-03-2019)

CCT8 band shows up prominently with no background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

CCT8 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:12263-1-AP)