CCNDBP1 Polyklonaler Antikörper

CCNDBP1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 12363-1-AP

Synonyme

HHM, Grap2 and cyclin-D-interacting protein, GCIP, DIP1, Cyclin-D1-binding protein 1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12363-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12363-1-AP (CCNDBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 12363-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12363-1-AP (CCNDBP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 12363-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12363-1-AP (CCNDBP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 12363-1-AP

  Immunofluorescent analysis of Hela cells, using CCNDBP1 antibody 12363-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

12363-1-AP bindet in WB, IHC, CoIP, ELISA CCNDBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCNDBP1 fusion protein Ag3032
• Vollständiger Name: cyclin D-type binding-protein 1
• Berechnetes Molekulargewicht: 360 aa, 40 kDa
• GenBank-Zugangsnummer: BC009689
• Gene symbol: CCNDBP1
• Gene ID (NCBI): 23582
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCNDBP1, also named as DIP1, GCIP and HHM, is a helix-loop-helix protein which suppresses tumorigenesis. CCNDBP1 specifically interacts with one of the class III HDAC proteins, SirT6, which is important for maintaining genome stability. CCNDBP1 may negatively regulate cell cycle progression.

Publikationen

Species	Application	Title
rat	WB	Biomed Pharmacother; Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ.; Authors - Ji-Ru Zhang
human	WB,IHC	Front Oncol; CCNDBP1, a Prognostic Marker Regulated by DNA Methylation, Inhibits Aggressive Behavior in Dedifferentiated Liposarcoma via Repressing Epithelial Mesenchymal Transition; Authors - Lingge Yang
human	WB,CoIP	J Cell Mol Med; LncRNA ABHD11-AS1 promotes the development of endometrial carcinoma by targeting cyclin D1.; Authors - Yao Liu
human	WB	Oncol Rep; Expression and prognostic significance of TRPV6 in the development and progression of pancreatic cancer.; Authors - He Song
human	WB	Am J Transl Res; Lithium chloride inhibits cell survival, overcomes drug resistance, and triggers apoptosis in multiple myeloma via activation of the Wnt/β-catenin pathway.; Authors - Ruosi Yao
		Oncol Rep; HOXA5 is a tumor suppressor gene that is decreased in gastric cancer.; Authors - Xudong Peng