Cyclin B2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Cyclin B2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G8B7

Kat-Nr. : 67726-1-Ig

Synonyme

CCNB2, Cyclin B, cyclin B2, G2/mitotic specific cyclin B2, HsT17299

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Galerie der Validierungsdaten

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  WB analysis using 67726-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67726-1-Ig (CCNB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67726-1-Ig

  WB result of Cyclin B2 antibody (67726-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B2 transfected HeLa cells.

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  IHC staining of human breast cancer using 67726-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67726-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 67726-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse testis using 67726-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of PC-12 using 67726-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushodengewebe, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67726-1-Ig bindet in WB, IHC, IF, ELISA Cyclin B2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cyclin B2 fusion protein Ag16329
• Vollständiger Name: cyclin B2
• Berechnetes Molekulargewicht: 398 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC105086
• Gene symbol: CCNB2
• Gene ID (NCBI): 9133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.