MIP-3 Beta Polyklonaler Antikörper

MIP-3 Beta Polyklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13397-1-AP

Synonyme

CCL19, MIP-3-beta, CCL=19, CCL 19, C-C motif chemokine 19

---

Galerie der Validierungsdaten

• 

  WB analysis using 13397-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 13397-1-AP (MIP-3 Beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 13397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MIP-3 Beta antibody (13397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of A549 using 13397-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 13397-1-AP (MIP-3-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 13397-1-AP

  Immunofluorescent analysis of A549 cells using 13397-1-AP (MIP-3-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A549 using 13397-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human MIP-3 Beta (13397-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

13397-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA MIP-3 Beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIP-3 Beta fusion protein Ag4200
• Vollständiger Name: chemokine (C-C motif) ligand 19
• Berechnetes Molekulargewicht: 98 aa, 12 kDa
• GenBank-Zugangsnummer: BC027968
• Gene symbol: CCL19/MIP-3 beta
• Gene ID (NCBI): 6363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

Publikationen

Species	Application	Title
human	IF	Am J Respir Crit Care Med; Pulmonary lymphoid neogenesis in idiopathic pulmonary arterial hypertension.; Authors - Perros Frédéric F
human	IF	Ann Rheum Dis; Global chemokine expression in systemic sclerosis (SSc): CCL19 expression correlates with vascular inflammation in SSc skin.; Authors - Allison L Mathes
human	IF	Front Immunol; The Impact of Immune Microenvironment on the Prognosis of Pancreatic Ductal Adenocarcinoma Based on Multi-Omics Analysis; Authors - Bing Yang
human	IHC	J Innate Immun; The Gene Signature of Activated M-CSF-Primed Human Monocyte-Derived Macrophages Is IL-10-Dependent; Authors - Víctor D. Cuevas
human	IHC	Front Physiol; Potential biomarkers and immune cell infiltration involved in aortic valve calcification identified through integrated bioinformatics analysis; Authors - Xiaoshuo Lv
human	IHC	Front Oncol; CXCL12, a potential modulator of tumor immune microenvironment (TIME) of bladder cancer: From a comprehensive analysis of TCGA database; Authors - Jinyan Wang