CCDC6 Polyklonaler Antikörper

CCDC6 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 13717-1-AP

Synonyme

Papillary thyroid carcinoma-encoded protein, Coiled-coil domain-containing protein 6, coiled coil domain containing 6

---

Galerie der Validierungsdaten

• 

  WB analysis using 13717-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13717-1-AP (CCDC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 13717-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 13717-1-AP (CCDC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 13717-1-AP

  Immunofluorescent analysis of MCF-7 cells using 13717-1-AP (CCDC6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

13717-1-AP bindet in WB, IF/ICC, ELISA CCDC6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC6 fusion protein Ag4658
• Vollständiger Name: coiled-coil domain containing 6
• Berechnetes Molekulargewicht: 474 aa, 53 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC036757
• Gene symbol: CCDC6
• Gene ID (NCBI): 8030
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Circular RNA circDNA2 upregulates CCDC6 expression to promote the progression of gastric cancer via miR-149-5p suppression.; Authors - Duochen Jin
human	WB	Mol Cancer Res; Dominant-Negative ATF5 Compromises Cancer Cell Survival by Targeting CEBPB and CEBPD.; Authors - Xiaotian Sun
human	WB	Genes (Basel); Insights into the Evolution of Spermatogenesis-Related Ubiquitin-Proteasome System Genes in Abdominal Testicular Laurasiatherians.; Authors - Xiaoyue Ding
rat	WB	Brain Res Bull; Attenuation of histone H4 lysine 16 acetylation (H4K16ac) elicits a neuroprotection against ischemic stroke by alleviating the autophagic/lysosomal dysfunction in neurons at the penumbra.; Authors - Dong Lingling
human	WB	Anal Bioanal Chem; A gene-encoded FRET fluorescent sensor designed for detecting asymmetric dimethylation levels in vitro and in living cells; Authors - Xuan Sun
human	WB	World J Gastroenterol; Suberoylanilide hydroxamic acid upregulates reticulophagy receptor expression and promotes cell death in hepatocellular carcinoma cells; Authors - Jia-Yao Li