CCBL1/KAT1 Polyklonaler Antikörper

CCBL1/KAT1 Polyklonal Antikörper für WB, IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 12156-1-AP

Synonyme

CCBL1, CCBL1/KAT1, Glutamine transaminase K, GTK, KAT1, KATI, KYAT1, Kynurenine aminotransferase I

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 12156-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12156-1-AP (CCBL1/KAT1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12156-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12156-1-AP (CCBL1/KAT1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12156-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12156-1-AP (CCBL1/KAT1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 12156-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12156-1-AP (CCBL1/KAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HeLa-Zellen, L02-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 1 publications below

Produktinformation

12156-1-AP bindet in WB, IF, IHC, ELISA CCBL1/KAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCBL1/KAT1 fusion protein Ag2828
• Vollständiger Name: cysteine conjugate-beta lyase, cytoplasmic
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC013069
• Gene symbol: CCBL1
• Gene ID (NCBI): 883
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCBL1 encodes kynurenine aminotransferase 1 (KAT1), a key enzyme involved in kynurenine pathway. CCBL1 catalyzes the production of kynurenic acid, a powerful endogenous excitatory amino acid receptor antagonist that is widely regarded as the potent neuroprotective agent.

Publikationen

Species	Application	Title
mouse	WB	Cell; Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience to stress-induced depression.; Authors - Leandro Z Agudelo
human	WB,IHC	J Intern Med; Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans.; Authors - R Baumgartner
mouse	WB	Brain Behav Immun; Kynurenine pathway is altered in BDNF Val66Met knock-in mice: Effect of physical exercise.; Authors - Alessandro Ieraci
		Am J Physiol Cell Physiol; Endurance exercise increases skeletal muscle kynurenine aminotransferases and plasma kynurenic acid in humans.; Authors - Maja Schlittler
mouse	WB	Sci Rep; Caffeine protects against stress-induced murine depression through activation of PPARγC1α-mediated restoration of the kynurenine pathway in the skeletal muscle.; Authors - Chongye Fang
human	WB	Exp Gerontol; Kynurenine aminotransferase isoforms display fiber-type specific expression in young and old human skeletal muscle.; Authors - V L Wyckelsma