CC2D1A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CC2D1A Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16816-1-AP

Synonyme

CC2D1A, FREUD 1, Freud 1/Aki1, MRT3

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 16816-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16816-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16816-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16816-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16816-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16816-1-AP

  IP Result of anti-CC2D1A (IP:16816-1-AP, 4ug; Detection:16816-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human testis using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16816-1-AP

  Immunofluorescent analysis of HepG2 cells, using CC2D1A antibody 16816-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

16816-1-AP bindet in WB, IP, IHC, IF, ELISA CC2D1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CC2D1A fusion protein Ag10355
• Vollständiger Name: coiled-coil and C2 domain containing 1A
• Berechnetes Molekulargewicht: 951 aa, 104 kDa
• Beobachtetes Molekulargewicht: 130 kDa, 104 kDa
• GenBank-Zugangsnummer: BC064981
• Gene symbol: CC2D1A
• Gene ID (NCBI): 54862
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CC2D1A (coiled-coil and C2 domain-containing 1A), also known as Freud-1, Aki1 or TAPE (TBK1-associated protein in endolysosomes), is a evolutionary conserved protein located in different subcellular compartments, including the nucleus, centrosome, and endolysosomes. It acts as a scaffold protein that interacts with various proteins and plays diverse biological roles. Mutations in CC2D1A have been linked to nonsyndromic mental retardation (NMSR) and generate a truncated 85-kDa product. Several isoforms of CC2D1A proteins have also been described: 120 /130-kDa doublet of long isoform and 67 kDa short isoform. The short isoform of CC2D1A has been identified as the predominant isoform in rodent cells, while the long isoform is more abundant in human cells. Recently it has been reported that CC2D1A/TAPE is the first innate immune regulator implicated in both TLR and RLR signaling at a very early step.

Publikationen

Species	Application	Title
rat	WB	Anesthesiology; Astroglial MicroRNA-219-5p in the Ventral Tegmental Area Regulates Nociception in Rats.; Authors - Song Zhang
mouse	WB	J Biol Chem; TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity.; Authors - Chen Kuan-Ru KR ; KD Validated
mouse	WB	Neurotherapeutics; Loss of CC2D1A in Glutamatergic Neurons Results in Autistic-Like Features in Mice.; Authors - Cheng-Yi Yang
mouse	WB	J Neurosci; Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation.; Authors - Cheng-Yi Yang